Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 13444-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 13444-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 13444-1-AP (DVL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Raji using 13444-1-AP
IP result of anti-DVL3 (IP:13444-1-AP, 4ug; Detection:13444-1-AP 1:500) with Raji cells lysate 1440ug.
IHC staining of human colon cancer using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 13444-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 13444-1-AP (DVL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 13444-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DVL3 antibody (13444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 13444-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13444-1-AP (DVL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DVL3 Polyclonal antibody

DVL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13444-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Dishevelled 3, Segment polarity protein dishevelled homolog DVL-3, KIAA0208, DSH homolog 3, Dishevelled-3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, MCF-7 cells, Raji cells
Positive IP detected in	Raji cells
Positive IHC detected in	human colon cancer tissue, human prostate cancer tissue, mouse colon tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 2 publications below
IF	See 2 publications below
IP	See 2 publications below

Product Information

13444-1-AP targets DVL3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, xenopus
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DVL3 fusion protein Ag4272 相同性解析による交差性が予測される生物種
Full Name	dishevelled, dsh homolog 3 (Drosophila)
Calculated molecular weight	716 aa, 78 kDa
Observed molecular weight	78 kDa
GenBank accession number	BC032459
Gene Symbol	DVL3
Gene ID (NCBI)	1857
RRID	AB_2093451
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92997
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for DVL3 antibody 13444-1-AP	Download protocol
IHC protocol for DVL3 antibody 13444-1-AP	Download protocol
IF protocol for DVL3 antibody 13444-1-AP	Download protocol
IP protocol for DVL3 antibody 13444-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
Xenopus	WB,IP	J Cell Biol Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled. Authors - Moonsup Lee View Article
mouse	IF	J Cell Biol Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling. Authors - Andre Landin Malt View Article
human	WB	Oncogene The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer. Authors - Kai Li View Article
human	WB	Cancer Lett SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network. Authors - Li Xuefei X View Article
human	WB	Oncotarget Dalbinol, a rotenoid from Amorpha fruticosa L., exerts anti-proliferative activity by facilitating β-catenin degradation in hepatocellular carcinoma cells. Authors - Xiaohui Zhu View Article
human	WB,IHC,IF	Biol Proced Online Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3 Authors - Mengqi Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13444-1-AP

WB analysis of MCF-7 using 13444-1-AP

IP experiment of Raji using 13444-1-AP

IHC staining of human colon cancer using 13444-1-AP

IHC staining of human colon cancer using 13444-1-AP

IHC staining of human cervical cancer using 13444-1-AP

IHC staining of human cervical cancer using 13444-1-AP

IHC staining of human prostate cancer using 13444-1-AP

IHC staining of human prostate cancer using 13444-1-AP

IHC staining of mouse colon using 13444-1-AP

IHC staining of mouse colon using 13444-1-AP

IF Staining of HeLa using 13444-1-AP

IF Staining of HeLa using 13444-1-AP

DVL3 Polyclonal antibody

DVL3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled.

Wnts regulate planar cell polarity via heterotrimeric G protein and PI3K signaling.

The Wnt/β-catenin/VASP positive feedback loop drives cell proliferation and migration in breast cancer.

SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network.

Dalbinol, a rotenoid from Amorpha fruticosa L., exerts anti-proliferative activity by facilitating β-catenin degradation in hepatocellular carcinoma cells.

Circadian gene CSNK1D promoted the progression of hepatocellular carcinoma by activating Wnt/β-catenin pathway via stabilizing Dishevelled Segment Polarity Protein 3