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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3C4

Cat no : 66008-3-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-3-Ig)

    WB analysis of HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

    HEK-293 and DDDDK-DPM1 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-3-Ig (DDDDK tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-3-Ig)

    IP experiment of Transfected HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

    IP result of anti-DYKDDDDK tag (IP:66008-3-Ig, 5ug; Detection:66008-3-Ig 1:1000) with Transfected HEK-293 cells lysate 1600 ug. This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-3-Ig)

    IF Staining of Transfected HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-3-Ig)

    IF Staining of Transfected HEK-293 using 66008-3-Ig (same clone as 66008-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 66008-3-Ig (DDDDK tag antibody) at dilution of 1:400 and Alexa Fluor 594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-3-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66008-3-PBS targets DYKDDDDK tag in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
RRIDAB_2749837
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.