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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    HEK-293 and different loading amount of DYKDDDDK tagged TDP-43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    HEK-293 and DYKDDDDK-TDP43 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDK tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    WB analysis using 66008-4-Ig (same clone as 66008-4-PBS)

    HEK-293 and DYKDDDDK-PCSK9 transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 66008-4-Ig (DYKDDDDKtag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.The membrane was re-blotted with GAPDH antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    IP experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    IP result of anti-DYKDDDDK tag (IP:66008-4-Ig, 5 ug; Detection:80010-1-RR 1:10000) with Transfected HEK-293T cells lysate 250 ug. This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    IF Staining of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed DYKDDDDK-TDP43 Transfected HEK-293 cells using DYKDDDDK tag antibody (66008-4-Ig, Clone: 8H6A10 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using DYKDDDDK tag Monoclonal antibody (Binds to FLAG® t (66008-4-Ig)

    FC experiment of Transfected HEK-293 using 66008-4-Ig (same clone as 66008-4-PBS)

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug DYKDDDDK tag Monoclonal antibody (Binds to FLAG® tag epitope) (66008-4-Ig, Clone:8H6A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66008-4-PBS in a different storage buffer formulation.

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DYKDDDDK tag Monoclonal antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 66008-4-PBS
Clone No.8H6A10

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Flag, DYKDDDDK, DDDDK tag, DDDDK

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66008-4-PBS targets DYKDDDDK tag in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Flag Tag
Gene Symbol Flag Tag
Gene ID (NCBI) 8
RRIDAB_2918475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DKYDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.