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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DYKDDDDK tag Recombinant antibody, PBS Only (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K14

Cat no : 80010-1-PBS

Synonyms

DDDDK, Flag tag, Flag, DYKDDDDK, DDDDK tag

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using DYKDDDDK tag Recombinant antibody (Binds to FLAG® (80010-1-RR)

    IP experiment of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    IP result of anti-DYKDDDDK tag (IP:80010-1-RR, 4ug; Detection:80010-1-RR 1:3000) with Transfected HEK-293 cells lysate 400 ug. This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DYKDDDDK tag Recombinant antibody (Binds to FLAG® (80010-1-RR)

    IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 (eGFP-DYKDDDDK transfected) cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), eGFP (Green), DYKDDDDK (Red), DAPI (Blue). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Recombinant antibody (Binds to FLAG® (80010-1-RR)

    IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Recombinant antibody (Binds to FLAG® (80010-1-RR)

    IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DDDDK tag antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using DYKDDDDK tag Recombinant antibody (Binds to FLAG® (80010-1-RR)

    IF Staining of Transfected HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using 80010-1-RR (DYKDDDDK tag antibody), at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using DYKDDDDK tag Recombinant antibody (Binds to FLAG® (80010-1-RR)

    IF Staining of HEK-293 using 80010-1-RR (same clone as 80010-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DYKDDDDK tag antibody (80010-1-RR, Clone: 4K14 ) at dilution of 1:500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), GFP (green). This data was developed using the same antibody clone with 80010-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80010-1-PBS targets DYKDDDDK tag in IF/ICC, IP, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329 相同性解析による交差性が予測される生物種
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
RRIDAB_2882940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

DYKDDDDK Tag (Equivalent To FLAG Antibody From Sigma) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DYKDDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.