DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
240568A11
Cat no : 80801-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | TDP-43-flag Transfected HEK-293T cells |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF/ICC detected in | Transfected HEK-293T cells |
Positive FC (Intra) detected in | HEK-293T transfected with TDP-43-flag |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 80801-2-RR is DYKDDDDK tag Fusion Protein expressed in E. coli.
Tested Reactivity | recombinant protein |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | DYKDDDDK tag fusion protein Ag2329 相同性解析による交差性が予測される生物種 |
Full Name | DYKDDDDK tag |
Gene symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
Protocols
Product Specific Protocols | |
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WB protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
IF protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
IP protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
FC protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |