DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)

DYKDDDDK tag Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

240568A11

Cat no : 80801-2-RR

Synonyms

3Flag, Flag, DDDDKtag, DDDDK tag, DDDDK


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Tested Applications

Positive WB detected inTDP-43-flag Transfected HEK-293T cells
Positive IP detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293T cells
Positive FC (Intra) detected inHEK-293T transfected with TDP-43-flag

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 80801-2-RR is DYKDDDDK tag Fusion Protein expressed in E. coli.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DYKDDDDK tag fusion protein Ag2329 相同性解析による交差性が予測される生物種
Full Name DYKDDDDK tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit polyclonal antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Protocols

Product Specific Protocols
WB protocol for DYKDDDDK tag antibody 80801-2-RRDownload protocol
IF protocol for DYKDDDDK tag antibody 80801-2-RRDownload protocol
IP protocol for DYKDDDDK tag antibody 80801-2-RRDownload protocol
FC protocol for DYKDDDDK tag antibody 80801-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols