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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dymeclin Polyclonal antibody (12001-1-AP)

    WB analysis using 12001-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12001-1-AP (Dymeclin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Dymeclin Polyclonal antibody

Dymeclin Polyclonal Antibody for WB, ELISA
Cat No. 12001-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DMC, DYM, Dymeclin, SMC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse kidney tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12001-1-AP targets Dymeclin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dymeclin fusion protein Ag2600 相同性解析による交差性が予測される生物種
Full Name dymeclin
Calculated molecular weight 669 aa, 76 kDa
Observed molecular weight 76-80 kDa
GenBank accession numberBC001252
Gene Symbol Dymeclin
Gene ID (NCBI) 54808
RRIDAB_2246056
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7RTS9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human Dymeclin is encoded by DYM gene, defects in which are the cause of Dyggve-Melchior-Clausen syndrome (DMC) and Smith-McCort dysplasia (SMC). DMC is a rare autosomal recessive disorder characterized by short trunk dwarfism, microcephaly and psychomotor retardation, with cutaneous cells containing dilated rough endoplasmic reticulum, enlarged and aberrant vacuoles and numerous vesicles [PubMed: 12491225]. Distinct with features of DMS, SMC is a rare osteochondrodysplasia characterized by short limbs and trunk with barrel-shaped chest [PubMed: 19005420]. Dymeclin is a not a stably anchored transmembrane protein with a transmembrane domain, but it shuttles between Golgi and cytosol, which is necessary for correct organization of Golgi apparatus [PubMed: 18996921].

Protocols

Product Specific Protocols
WB protocol for Dymeclin antibody 12001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols