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  • Featured Product
  • KD/KO Validated

DYNC1I2 Polyclonal antibody

DYNC1I2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12219-1-AP

Synonyms

IC2, DNCIC2, DNCI2, DH IC-2, DH IC 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of Jurkat using 12219-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of A375 using 12219-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of human brain using 12219-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of mouse testis using 12219-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IP experiment of mouse testis using 12219-1-AP

    IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IP experiment of HeLa using 12219-1-AP

    IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IHC staining of mouse stomach using 12219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IHC staining of mouse stomach using 12219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IHC staining of mouse cerebellum using 12219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IHC staining of mouse cerebellum using 12219-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IHC staining of rat cerebellum using 12219-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IHC staining of rat cerebellum using 12219-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IF Staining of A375 using 12219-1-AP

    Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, A375 cells, human brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue, HeLa cells
Positive IHC detected inmouse stomach tissue, mouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12219-1-AP targets DYNC1I2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNC1I2 fusion protein Ag2873 相同性解析による交差性が予測される生物種
Full Name dynein, cytoplasmic 1, intermediate chain 2
Calculated molecular weight 612 aa, 68 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC015038
Gene symbol DYNC1I2
Gene ID (NCBI) 1781
RRIDAB_2093630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IHC protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IF protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IP protocol for DYNC1I2 antibody 12219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Authors - Hironori Inaba
  • KD Validated
View Article
humanIP

Development

SPEF2 functions in microtubule-mediated transport in elongating spermatids.

Authors - Mari S Lehti
View Article