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  • Featured Product
  • KD/KO Validated

DYNC1I2 Polyclonal antibody

DYNC1I2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12219-1-AP

Synonyms

IC2, DNCIC2, DNCI2, DH IC-2, DH IC 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of Jurkat using 12219-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of A375 using 12219-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of human brain using 12219-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    WB analysis of mouse testis using 12219-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12219-1-AP (DYNC1I2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IP experiment of mouse testis using 12219-1-AP

    IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IP experiment of HeLa using 12219-1-AP

    IP result of anti-DYNC1I2 (IP:12219-1-AP, 4ug; Detection:12219-1-AP 1:500) with HeLa cells lysate 2080 ug.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using DYNC1I2 Polyclonal antibody (12219-1-AP)

    IF Staining of A375 using 12219-1-AP

    Immunofluorescent analysis of A375 cells using 12219-1-AP (DYNC1I2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, A375 cells, human brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue, HeLa cells
Positive IF/ICC detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12219-1-AP targets DYNC1I2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNC1I2 fusion protein Ag2873 相同性解析による交差性が予測される生物種
Full Name dynein, cytoplasmic 1, intermediate chain 2
Calculated molecular weight 612 aa, 68 kDa
Observed molecular weight 68-72 kDa
GenBank accession numberBC015038
Gene symbol DYNC1I2
Gene ID (NCBI) 1781
RRIDAB_2093630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IF protocol for DYNC1I2 antibody 12219-1-APDownload protocol
IP protocol for DYNC1I2 antibody 12219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Authors - Hironori Inaba
  • KD Validated
View Article
humanIP

Development

SPEF2 functions in microtubule-mediated transport in elongating spermatids.

Authors - Mari S Lehti
View Article