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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DYNC2H1 Polyclonal antibody

DYNC2H1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29758-1-AP

Synonyms

DHC1b, DHC2, DNCH2, DYH1B, DYNC2H1, FLJ11756, hdhc11

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using DYNC2H1 Polyclonal antibody (29758-1-AP)

    WB analysis of mouse testis using 29758-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 29758-1-AP (DYNC2H1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IHC staining of mouse kidney using 29758-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IHC staining of mouse kidney using 29758-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29758-1-AP (DYNC2H1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IF Staining of hTERT-RPE1 using 29758-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using DYNC2H1 Polyclonal antibody (29758-1-AP)

    IF Staining of hTERT-RPE1 using 29758-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DYNC2H1 antibody (29758-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29758-1-AP targets DYNC2H1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNC2H1 fusion protein Ag30707 相同性解析による交差性が予測される生物種
Full Name dynein, cytoplasmic 2, heavy chain 1
Calculated molecular weight 493 kDa
Observed molecular weight493 kDa
GenBank accession numberNM_001377
Gene symbol DYNC2H1
Gene ID (NCBI) 79659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DYNC2H1 (Cytoplasmic dynein 2 heavy chain 1) is involved in ciliary intraflagellar transport (IFT). DYNC2H1 drives retrograde transport of the IFT-A protein complex that regulates tip-to-base transport in cilia, involved in the generation and maintenance of cilia. DYNC2H1 variants or retina-predominant variants cause nonsyndromic retinal degeneration (PMID:32753734). DYNC2H1 mutations also cause asphyxiating thoracic dystrophy and short rib-polydactyly syndrome (PMID: 19442771). DYNC2H1 was localized to the basal bodies by immunofluorescence staining (PMID: 21552265, PMID: 26077881, PMID: 30320547).

Protocols

Product Specific Protocols
WB protocol for DYNC2H1 antibody 29758-1-APDownload protocol
IHC protocol for DYNC2H1 antibody 29758-1-APDownload protocol
IF protocol for DYNC2H1 antibody 29758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols