DYNC2LI1 Polyclonal antibody
DYNC2LI1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 15949-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, L02 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 2 publications below |
Product Information
15949-1-AP targets DYNC2LI1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DYNC2LI1 fusion protein Ag8584 相同性解析による交差性が予測される生物種 |
| Full Name | dynein, cytoplasmic 2, light intermediate chain 1 |
| Calculated molecular weight | 352 aa, 40 kDa |
| Observed molecular weight | 37-40 kDa, 22 kDa |
| GenBank accession number | BC006969 |
| Gene symbol | DYNC2LI1 |
| Gene ID (NCBI) | 51626 |
| RRID | AB_2093643 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYNC2LI1 (D2LIC, LIC3) is a member of cytoplasmic dynein 2 complex that is required for retrograde transport. It is ubiquitously expressed with high expression levels in brain, kidney, lung and testes. In mammalian cells, DYNC2LI1 is localized to centrosomal region Mutations in DYNC2LI1 disrupt cilia function and cause various skeletal ciliopathies. There're some isoforms with MW 40 kDa, 38 kDa, 22 kDa and 16 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYNC2LI1 antibody 15949-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Dynein-2 intermediate chains play crucial but distinct roles in primary cilia formation and function. | ||
Hum Mol Genet Dynll1 is essential for development and promotes endochondral bone formation by regulating intraflagellar Dynein function in primary cilia. | ||
FEBS Lett Recombinant human cytoplasmic dynein heavy chain 1 and 2: observation of dynein-2 motor activity in vitro. | ||
Cilia Mutations in IFT-A satellite core component genes IFT43 and IFT121 produce short rib polydactyly syndrome with distinctive campomelia. | ||
