• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DYNLT3 Polyclonal antibody

DYNLT3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11687-1-AP

Synonyms

TCTEX1L, TCTE1XL, TCTE1L, T-complex-associated testis-expressed 1-like, RP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of U-937 cells using DYNLT3 Polyclonal antibody (11687-1-AP)

    WB analysis of U-937 using 11687-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 11687-1-AP (DYNLT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DYNLT3 Polyclonal antibody (11687-1-AP)

    WB analysis of HeLa using 11687-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11687-1-AP (DYNLT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DYNLT3 Polyclonal antibody (11687-1-AP)

    IHC staining of human ovary tumor using 11687-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11687-1-AP (DYNLT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using DYNLT3 Polyclonal antibody (11687-1-AP)

    IHC staining of human ovary tumor using 11687-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11687-1-AP (DYNLT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using DYNLT3 Polyclonal antibody (11687-1-AP)

    IF Staining of HepG2 using 11687-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DYNLT3 antibody (11687-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inU-937 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11687-1-AP targets DYNLT3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYNLT3 fusion protein Ag2299 相同性解析による交差性が予測される生物種
Full Name dynein, light chain, Tctex-type 3
Calculated molecular weight 116 aa, 13 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC000968
Gene symbol DYNLT3
Gene ID (NCBI) 6990
RRIDAB_2093777
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dynein light chain Tctex-type 3 (DYNLT3) is a member of the cytoplasmic dynamic protein light chain Tctex family. DYNLT3 mainly plays a regulatory role in mitosis and meiosis, as well as chromosome binding. DYNLT3 is expressed in all cells and tissues. The calculated molecular weight of DYNLT3 is 13 kDa.

Protocols

Product Specific Protocols
WB protocol for DYNLT3 antibody 11687-1-APDownload protocol
IHC protocol for DYNLT3 antibody 11687-1-APDownload protocol
IF protocol for DYNLT3 antibody 11687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols