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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using DYRK1A Polyclonal antibody (28607-1-AP)

    WB analysis of HEK-293 using 28607-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using DYRK1A Polyclonal antibody (28607-1-AP)

    WB analysis of PC-3 using 28607-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using DYRK1A Polyclonal antibody (28607-1-AP)

    WB analysis using 28607-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28607-1-AP (DYRK1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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DYRK1A Polyclonal antibody

DYRK1A Polyclonal Antibody for WB, ELISA
Cat No. 28607-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

DYRK, DYRK1, DYRK1A, hMNB, HP86, MNB, MNBH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28607-1-AP targets DYRK1A in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DYRK1A fusion protein Ag29433 相同性解析による交差性が予測される生物種
Full Name dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A
Calculated molecular weight 86 kDa
Observed molecular weight70kDa, 85 kDa
GenBank accession numberNM_001396
Gene Symbol DYRK1A
Gene ID (NCBI) 1859
RRIDAB_3086069
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13627
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome.

Protocols

Product Specific Protocols
WB protocol for DYRK1A antibody 28607-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols