Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat testis tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
14522-1-AP targets DYX1C1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DYX1C1 fusion protein Ag5997 相同性解析による交差性が予測される生物種 |
| Full Name | dyslexia susceptibility 1 candidate 1 |
| Calculated molecular weight | 49 kDa |
| Observed molecular weight | 48 kDa |
| GenBank accession number | BC062564 |
| Gene Symbol | DYX1C1 |
| Gene ID (NCBI) | 161582 |
| RRID | AB_2093955 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DYX1C1 initially was described as the first candidate gene for developmental dyslexia. The protein contains three TPR motifs and one p23 domain, all commonly known for their protein-protein interactions. It also has been linked to neuronal migration. Recently it has been reported that the expression of DYX1C1 protein is lost in breast cancer and patients who have lost DYX1C1 expression have a poor overall survival,indicating that DYX1C1 is a potential prognostic biomarker in breast cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DYX1C1 antibody 14522-1-AP | Download protocol |
| IP protocol for DYX1C1 antibody 14522-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet DYX1C1 is required for axonemal dynein assembly and ciliary motility.
| ||
Biol Psychiatry Molecular networks of DYX1C1 gene show connection to neuronal migration genes and cytoskeletal proteins. | ||
Mol Endocrinol The rs3743205 SNP is important for the regulation of the dyslexia candidate gene DYX1C1 by estrogen receptor β and DNA methylation. | ||
BMC Cancer The dyslexia candidate gene DYX1C1 is a potential marker of poor survival in breast cancer. | ||
BMC Mol Cell Biol Genetic and protein interaction studies between the ciliary dyslexia candidate genes DYX1C1 and DCDC2 |