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Validation Data Gallery

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  • Western Blot (WB) analysis of pig cartilage tissue using Decorin Monoclonal antibody (66847-1-Ig)

    WB analysis of pig cartilage using 66847-1-Ig (same clone as 66847-1-PBS)

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66847-1-Ig (Decorin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66847-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using Decorin Monoclonal antibody (66847-1-Ig)

    IF Staining of BxPC-3 using 66847-1-Ig (same clone as 66847-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Decorin antibody (66847-1-Ig, Clone: 3A4E4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66847-1-PBS in a different storage buffer formulation.

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Decorin Monoclonal antibody, PBS Only

Decorin Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66847-1-PBS
Clone No.3A4E4

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66847-1-PBS targets Decorin in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Decorin fusion protein Ag6275 相同性解析による交差性が予測される生物種
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight 40 kDa, 100 kDa
GenBank accession numberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
RRIDAB_2882187
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07585
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.