Decorin Recombinant antibody

Decorin Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240781E3

Cat no : 83753-4-RR

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD


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Tested Applications

Positive WB detected inMCF-7 cells, ROS1728 cells, mouse colon tissue
Positive FC (Intra) detected inHEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83753-4-RR is Decorin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Decorin fusion protein Ag6275 相同性解析による交差性が予測される生物種
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight70 kDa
GenBank accession numberBC005322
Gene symbol Decorin
Gene ID (NCBI) 1634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 83753-4-RRDownload protocol
FC protocol for Decorin antibody 83753-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols