Decorin Recombinant antibody
Decorin Recombinant Antibody for WB, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240781E3
Cat no : 83753-4-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, ROS1728 cells, mouse colon tissue |
Positive FC (Intra) detected in | HEK-293 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83753-4-RR is Decorin Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Decorin fusion protein Ag6275 相同性解析による交差性が予測される生物種 |
Full Name | decorin |
Calculated molecular weight | 359 aa, 40 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC005322 |
Gene symbol | Decorin |
Gene ID (NCBI) | 1634 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.
Protocols
Product Specific Protocols | |
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WB protocol for Decorin antibody 83753-4-RR | Download protocol |
FC protocol for Decorin antibody 83753-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |