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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Decorin Recombinant antibody (83753-4-RR)

    WB analysis using 83753-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using Decorin Recombinant antibody (83753-4-RR)

    FC experiment of HepG2 using 83753-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HEK-293 cells using Decorin Recombinant antibody (83753-4-RR)

    FC experiment of HEK-293 using 83753-4-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83753-4-RR

    Affinity and Kinetic Characterization of 83753-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

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Decorin Recombinant antibody

Decorin Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83753-4-RR
Clone No.240781E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, ROS1728 cells, mouse colon tissue
Positive FC (Intra) detected inHEK-293 cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83753-4-RR targets Decorin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Decorin fusion protein Ag6275 相同性解析による交差性が予測される生物種
Full Name decorin
Calculated molecular weight 359 aa, 40 kDa
Observed molecular weight70 kDa
GenBank accession numberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
RRIDAB_3671350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 83753-4-RRDownload protocol
FC protocol for Decorin antibody 83753-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols