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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skin tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    WB analysis of mouse skin using 10537-1-AP (same clone as 10537-1-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Dermatopontin Polyclonal antibody (10537-1-AP)

    WB analysis using 10537-1-AP (same clone as 10537-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skin tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    WB analysis of rat skin using 10537-1-AP (same clone as 10537-1-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    WB analysis of human skeletal muscle using 10537-1-AP (same clone as 10537-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10537-1-AP (Dermatopontin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of human pancreas using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of human pancreas using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of rat testis using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10537-1-AP (Dermatopontin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of rat testis using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10537-1-AP (Dermatopontin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of human heart using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of human heart using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of human testis using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of human testis using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of rat testis using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Dermatopontin Polyclonal antibody (10537-1-AP)

    IHC staining of rat testis using 10537-1-AP (same clone as 10537-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis using 10537-1-AP (Dermatopontin antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10537-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

Dermatopontin Polyclonal antibody, PBS Only

Dermatopontin Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10537-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10537-1-PBS targets Dermatopontin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dermatopontin fusion protein Ag0801 相同性解析による交差性が予測される生物種
Full Name dermatopontin
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC033736
Gene Symbol DPT/TRAMP
Gene ID (NCBI) 1805
RRIDAB_2230721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07507
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the growth inhibition activity of TGF-beta1.