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Validation Data Gallery

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  • Western Blot (WB) analysis of rat skin tissue using Dermatopontin Monoclonal antibody (66348-1-Ig)

    WB analysis of rat skin using 66348-1-Ig (same clone as 66348-1-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 66348-1-Ig (Dermatopontin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66348-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Dermatopontin Monoclonal antibody (66348-1-Ig)

    WB analysis of human testis using 66348-1-Ig (same clone as 66348-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66348-1-Ig (Dermatopontin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66348-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Dermatopontin Monoclonal antibody (66348-1-Ig)

    IF Staining of SH-SY5Y using 66348-1-Ig (same clone as 66348-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Dermatopontin antibody (66348-1-Ig, Clone: 4B1A12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66348-1-PBS in a different storage buffer formulation.

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Dermatopontin Monoclonal antibody, PBS Only

Dermatopontin Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66348-1-PBS
Clone No.4B1A12

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

dermatopontin, DPT, TRAMP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66348-1-PBS targets Dermatopontin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Dermatopontin fusion protein Ag6200 相同性解析による交差性が予測される生物種
Full Name dermatopontin
Calculated molecular weight 22 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC033736
Gene Symbol DPT/TRAMP
Gene ID (NCBI) 1805
RRIDAB_2881728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07507
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

DPT (dermatopontin) is an extracellular matrix protein belonging to the dermatopontin family. Skin appears to be the richest source of dermatopontin, comprising about 15 mg/kg of the wet weight. Expression of dermatopontin is not limited to connective tissue, as investigations show that dermatopontin mRNA is expressed in cultured fibroblasts, muscle, heart, pancreas, and lung. Dermatopontin comprises a considerable proportion of the noncollagenous extracellular matrix proteins. It is involved in promotion of cellular adhesion, extracellular matrix assembly, wound healing, and positive modification of the inhibition activity of TGF-beta1.