Validation Data Gallery

Filter: AllWBIHCIF-P

WB analysis using 67793-1-Ig (same clone as 67793-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
WB analysis of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 67793-1-Ig (same clone as 67793-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of human rectal cancer using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IHC staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IF Staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 67793-1-Ig (same clone as 67793-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.
IF Staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

Desmin Monoclonal antibody, PBS Only

Desmin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67793-1-PBS

Clone No.2B1A11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

DES, 2B1A11

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67793-1-PBS targets Desmin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity	human, mouse, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	Desmin fusion protein Ag9675 相同性解析による交差性が予測される生物種
Full Name	desmin
Calculated molecular weight	470 aa, 54 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC032116
Gene Symbol	Desmin
Gene ID (NCBI)	1674
RRID	AB_2918557
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P17661
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 67793-1-Ig (same clone as 67793-1-PBS)

WB analysis of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

WB analysis of mouse testis using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of human placenta using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of human rectal cancer using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of human placenta using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

IHC staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

IF Staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

IF Staining of human appendicitis using 67793-1-Ig (same clone as 67793-1-PBS)

IF Staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

Desmin Monoclonal antibody, PBS Only

Desmin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information