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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Desmin Monoclonal antibody, PBS Only

Desmin Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1A11

Cat no : 67793-1-PBS

Synonyms

Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin, FLJ12025, FLJ39719, FLJ41013, FLJ41793

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Desmin Monoclonal antibody (67793-1-Ig)

    WB analysis using 67793-1-Ig (same clone as 67793-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Desmin Monoclonal antibody (67793-1-Ig)

    WB analysis of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using Desmin Monoclonal antibody (67793-1-Ig)

    WB analysis of mouse testis using 67793-1-Ig (same clone as 67793-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 67793-1-Ig (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Desmin Monoclonal antibody (67793-1-Ig)

    IHC staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Desmin Monoclonal antibody (67793-1-Ig)

    IHC staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67793-1-Ig (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using Desmin Monoclonal antibody (67793-1-Ig)

    IF Staining of human colon using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using Desmin Monoclonal antibody (67793-1-Ig)

    IF Staining of human appendicitis using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Desmin Monoclonal antibody (67793-1-Ig)

    IF Staining of mouse skeletal muscle using 67793-1-Ig (same clone as 67793-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Desmin antibody (67793-1-Ig, Clone: 2B1A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67793-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67793-1-PBS targets Desmin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Desmin fusion protein Ag9675 相同性解析による交差性が予測される生物種
Full Name desmin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight52 kDa
GenBank accession numberBC032116
Gene symbol Desmin
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.