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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Desmin Recombinant antibody

Desmin Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D21

Cat no : 82748-1-RR

Synonyms

DES, 1D21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Desmin Recombinant antibody (82748-1-RR)

    WB analysis using 82748-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82748-1-RR (Desmin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Desmin Recombinant antibody (82748-1-RR)

    IHC staining of mouse skeletal muscle using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Desmin Recombinant antibody (82748-1-RR)

    IHC staining of mouse skeletal muscle using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human normal colon using Desmin Recombinant antibody (82748-1-RR)

    IHC staining of human normal colon using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Desmin Recombinant antibody (82748-1-RR)

    IHC staining of mouse heart using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Desmin Recombinant antibody (82748-1-RR)

    IHC staining of mouse heart using 82748-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82748-1-RR (Desmin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82748-1-RR

    Affinity and Kinetic Characterization of 82748-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82748-1-RR against Human Desmin were performed. The affinity constant is 1.32 nM.

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Tested Applications

Positive WB detected inC2C12 cells, pig heart tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82748-1-RR targets Desmin in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Desmin fusion protein Ag9742 相同性解析による交差性が予測される生物種
Full Name desmin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC032116
Gene symbol Desmin
Gene ID (NCBI) 1674
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

Protocols

Product Specific Protocols
WB protocol for Desmin antibody 82748-1-RRDownload protocol
IHC protocol for Desmin antibody 82748-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols