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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IF Staining of NIH/3T3 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IF Staining of C6 using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    IF Staining of HeLa using 84702-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    FC experiment of HeLa using 84702-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Dot Blot experiment of peptide using Di-Methyl-Histone H3 (Arg2) Recombinant antibody (84702-1-RR)

    Dot Blot experiment of peptide using 84702-1-RR

    Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Di-Methyl-Histone H3 (Arg2) Recombinant antibody

Di-Methyl-Histone H3 (Arg2) Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Dot Blot, ELISA
Cat No. 84702-1-RR
Clone No.241896D7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra), Dot Blot, ELISA

Synonyms

Di-Methyl-Histone H3, Di-Methyl Histone-H3 Arg2, Di-Methyl Histone H3 Arg2, Di-Methyl Histone H3 (Arg2), Di Methyl Histone H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected inHeLa cells
Positive Dot Blot detected inpeptide

This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3672118
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Di-Methyl-Histone H3 (Arg2) antibody 84702-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols