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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Di-Methyl-Histone H4 (Arg3) Recombinant antibody

Di-Methyl-Histone H4 (Arg3) Recombinant Antibody for WB, ELISA, Dot Blot

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA, Dot Blot

Conjugate

Unconjugated

CloneNo.

1E21

Cat no : 82859-1-RR

Synonyms

H4/a, H4/B, H4/C, H4/D, H4/E, H4/G, H4/H, H4/I, H4/J, H4/K, H4/M, H4/N, H4/O, H4F2, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4FO, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST2H4B, HIST4H4, histone cluster 1, H4a, Histone H4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    WB analysis using 82859-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    WB analysis of mouse liver using 82859-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment of peptide using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    Dot Blot experiment of peptide using 82859-1-RR

    Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inTHP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, ELISA, Dot Blot applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H4a
Observed molecular weight13 kDa
GenBank accession numberBC069654
Gene symbol HIST1H4A
Gene ID (NCBI) 8359
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols