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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Di-Methyl-Histone H4 (Arg3) Recombinant antibody

Di-Methyl-Histone H4 (Arg3) Recombinant Antibody for WB, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

1E21

Cat no : 82859-1-RR

Synonyms

HIST1H4A, 1E21, Histone-H4, histoneH4, Histone H4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    WB analysis using 82859-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    WB analysis of mouse liver using 82859-1-RR

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 82859-1-RR (Di-Methyl-Histone H4 (Arg3) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Dot Blot experiment of peptide using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    Dot Blot experiment of peptide using 82859-1-RR

    Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 5 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • Dot Blot experiment of peptide using Di-Methyl-Histone H4 (Arg3) Recombinant antibody (82859-1-RR)

    Dot Blot experiment of peptide using 82859-1-RR

    Dot blot analysis was used to confirm the specificity of 82859-1-RR Di-Methyl-Histone H4 (Arg3) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inTHP-1 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82859-1-RR targets Di-Methyl-Histone H4 (Arg3) in WB, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H4a
Observed molecular weight13 kDa
GenBank accession numberBC069654
Gene symbol HIST1H4A
Gene ID (NCBI) 8359
RRIDAB_3670583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Di-Methyl-Histone H4 (Arg3) antibody 82859-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols