• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antibody

Di/Tri-Methyl-Histone H3 (Lys36) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241139G6

Cat no : 84329-1-RR

Synonyms

HIST1H3A, 241139G6, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antib (84329-1-RR)

    WB analysis using 84329-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84329-1-RR (Di/Tri-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Histone H3 Recombinant antibody (84329-1-RR)

    IF Staining of HeLa using 84329-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84329-1-RR, Clone: 241139G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antib (84329-1-RR)

    Dot Blot experiment of peptide using 84329-1-RR

    Dot blot analysis was used to confirm the specificity of Di/Tri-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, U-87 MG cells, U-251 cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84329-1-RR targets Di/Tri-Methyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
Observed molecular weight15 kDa
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

Protocols

Product Specific Protocols
WB protocol for Di/Tri-Methyl-Histone H3 (Lys36) antibody 84329-1-RRDownload protocol
IF protocol for Di/Tri-Methyl-Histone H3 (Lys36) antibody 84329-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols