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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibody

Di/Tri-Methyl-Histone H3 (Lys4) Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

241942D11

Cat no : 84908-2-RR

Synonyms

HIST1H3A, 241942D11, Histone-H3, HistoneH3, Histone H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    WB analysis using 84908-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IHC staining of mouse testis using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IHC staining of mouse testis using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IHC staining of mouse liver using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IHC staining of mouse liver using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IHC staining of human placenta using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IHC staining of human placenta using 84908-2-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84908-2-RR (Di/Tri-Methyl-Histone H3 (Lys4) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    IF Staining of HeLa using 84908-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84908-2-RR, Clone: 241942D11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Di/Tri-Methyl-Histone H3 (Lys4) Recombinant antibo (84908-2-RR)

    Dot Blot experiment of peptide using 84908-2-RR

    Dot blot analysis was used to confirm the specificity of 84908-2-RR Di/Tri-Methyl-Histone H3 (Lys4) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

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Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue, mouse liver tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84908-2-RR targets Di/Tri-Methyl-Histone H3 (Lys4) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
GenBank accession numberBC066245
Gene symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RRDownload protocol
IHC protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RRDownload protocol
IF protocol for Di/Tri-Methyl-Histone H3 (Lys4) antibody 84908-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols