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ChromoTek Dnmt1-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagGFP2.

Specificity

Dmnt1

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Cat no : dcg

Synonyms

Chromobody, Dmnt1 Chromobody

Validation Data Gallery

  • Series of 4 images of HeLa cells expressing the Dnmt1-Chromobody (over 5.5 hours): In G phase cells, the signal is homogeneously distributed through the nucleus and cytoplasm. Over time, during S phase granules appear in the nucleus as replication foci form, finally the granularity disappears, and the cells divide.

    Series of 4 images of HeLa cells expressing the Dnmt1-Chromobody (over 5.5 hours): In G phase cells, the signal is homogeneously distributed through the nucleus and cytoplasm. Over time, during S phase granules appear in the nucleus as replication foci form, finally the granularity disappears, and the cells divide.

Product Information

DNA plasmid encoding for anti-Dnmt1 VHH (anti-Dnmt1 Nanobody) fused to TagGFP2.

DescriptionThe Dnmt1-Chromobody visualizes endogenous Dnmt1 in real-time during cell cycle progression.

• Trace dynamic changes during the cell cycle in real time

• Monitor the distribution of an endogenous cell cycle marker protein without overexpression artifacts or cytotoxic effects from overexpressed fluorescent protein fusion Dnmt1

ApplicationsLive cell imaging
SpecificityDnmt1 (DNA (cytosine-5)-methyltransferase 1), tested in human cells
ReporterTagGFP2
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
SequenceWith the Cell Cycle-Chromobody plasmid you receive the sequence information of the Alpaca antibody to PCNA fused to TagRFP, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED and 3D-SIM; high-content microscopy and analysis
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Commun

Nanobody-mediated control of gene expression and epigenetic memory.

Authors - Mike V Van
View Article

Biotechnol J

Tracing endogenous proteins in living cells through electrotransfer of mRNA encoding chromobodies

Authors - Théo Juncker
View Article