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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25770-1-AP

Synonyms

D0S117E, DBN1, Drebrin, drebrin 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Drebrin Polyclonal antibody (25770-1-AP)

    WB analysis using 25770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Drebrin Polyclonal antibody (25770-1-AP)

    WB analysis of K-562 using 25770-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using Drebrin Polyclonal antibody (25770-1-AP)

    WB analysis of Neuro-2a using 25770-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using Drebrin Polyclonal antibody (25770-1-AP)

    WB analysis of SH-SY5Y using 25770-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Drebrin Polyclonal antibody (25770-1-AP)

    WB analysis of mouse brain using 25770-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Drebrin Polyclonal antibody (25770-1-AP)

    IF Staining of SH-SY5Y using 25770-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using Drebrin Polyclonal antibody (25770-1-AP)

    IF Staining of MCF-7 using 25770-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Drebrin antibody (25770-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Drebrin Polyclonal antibody (25770-1-AP)

    IF Staining of HepG2 using 25770-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, SH-SY5Y cells, K-562 cells, Neuro-2a cells, Jurkat cells
Positive IF/ICC detected inMCF-7 cells, SH-SY5Y cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

25770-1-AP targets Drebrin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Drebrin fusion protein Ag22634 相同性解析による交差性が予測される生物種
Full Name drebrin 1
Observed molecular weight 100-130 kDa
GenBank accession numberBC007567
Gene symbol Drebrin
Gene ID (NCBI) 1627
RRIDAB_2880234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

Protocols

Product Specific Protocols
WB protocol for Drebrin antibody 25770-1-APDownload protocol
IF protocol for Drebrin antibody 25770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery

Authors - Paloma Alvarez-Suarez
View Article