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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Drebrin Recombinant antibody, PBS Only (Capture)

Drebrin Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240599G12

Cat no : 83591-2-PBS

Synonyms

DBN1, drebrin 1, Developmentally-regulated brain protein, DBN 1, 240599G12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Drebrin Recombinant antibody (83591-2-RR)

    WB analysis using 83591-2-RR (same clone as 83591-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83591-2-RR (Drebrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Drebrin Recombinant antibody (83591-2-RR)

    IHC staining of human stomach cancer using 83591-2-RR (same clone as 83591-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83591-2-RR (Drebrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Drebrin Recombinant antibody (83591-2-RR)

    FC experiment of SH-SY5Y using 83591-2-RR (same clone as 83591-2-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Drebrin Recombinant antibody (83591-2-RR, Clone:240599G12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83591-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00585-2

    Cytometric bead array standard curve of MP00585-2

    Cytometric bead array standard curve of MP00585-2, Drebrin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83591-2-PBS. Detection antibody: 83591-1-PBS. Standard: Ag0371. Range: 0.78-100 ng/mL.

  • Affinity and Kinetic Characterization of 83591-2-RR

    Affinity and Kinetic Characterization of 83591-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83591-2-RR against Human DHRS3 were performed. The affinity constant is 0.109 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83591-2-PBS targets Drebrin as part of a matched antibody pair:

MP00585-2: 83591-2-PBS capture and 83591-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Drebrin fusion protein Ag0371 相同性解析による交差性が予測される生物種
Full Name drebrin 1
Calculated molecular weight 71 kDa
Observed molecular weight 110-130 kDa
GenBank accession numberBC000283
Gene symbol Drebrin
Gene ID (NCBI) 1627
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.