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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Dynamitin (1-401 AA) Monoclonal antibody, PBS Only

Dynamitin (1-401 AA) Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B9A5

Cat no : 60257-1-PBS

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, Dynamitin, Dynamitin (1-401 AA), p50 dynamitin, RBP50

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using Dynamitin (1-401 AA) Monoclonal antibody (60257-1-Ig)

    WB analysis of human brain using 60257-1-Ig (same clone as 60257-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60257-1-Ig (Dynamitin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Dynamitin (1-401 AA) Monoclonal antibody (60257-1-Ig)

    IHC staining of human gliomas using 60257-1-Ig (same clone as 60257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60257-1-Ig (Dynamitin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using Dynamitin (1-401 AA) Monoclonal antibody (60257-1-Ig)

    IHC staining of human gliomas using 60257-1-Ig (same clone as 60257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas slide using 60257-1-Ig (Dynamitin antibody at dilution of 1:50. This data was developed using the same antibody clone with 60257-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60257-1-PBS targets Dynamitin (1-401 AA) in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Dynamitin (1-401 AA) fusion protein Ag1902 相同性解析による交差性が予測される生物種
Full Name dynactin 2 (p50)
Calculated molecular weight 401 aa, 44 kDa
Observed molecular weight 52+150 kDa
GenBank accession numberBC014083
Gene symbol Dynamitin
Gene ID (NCBI) 10540
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is is against the N terminal 86aa of full length p50 dynamitin.