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  • Featured Product
  • KD/KO Validated

E-cadherin Monoclonal antibody, PBS Only

E-cadherin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B11F11

Cat no : 60335-1-PBS

Synonyms

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using E-cadherin Monoclonal antibody (60335-1-Ig)

    WB analysis of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

    WB result of E-cadherin antibody (60335-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E-cadherin transfected A431 cells. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using E-cadherin Monoclonal antibody (60335-1-Ig)

    WB analysis of PC-3 using 60335-1-Ig (same clone as 60335-1-PBS)

    PC-3, MKN-45, SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using E-cadherin Monoclonal antibody (60335-1-Ig)

    WB analysis of A431 using 60335-1-Ig (same clone as 60335-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using E-cadherin Monoclonal antibody (60335-1-Ig)

    WB analysis of MCF-7 using 60335-1-Ig (same clone as 60335-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    WB analysis of pig brain using 60335-1-Ig (same clone as 60335-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60335-1-Ig (E-cadherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of rat stomach using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of rat stomach using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human colon using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human colon using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of rat colon using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of rat colon using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 60335-1-Ig (E-cadherin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IHC staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60335-1-Ig (E-cadherin Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IF Staining of human kidney using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:300 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), (18150-1-AP, green). DNA was stained by DAPI (blue). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using E-cadherin Monoclonal antibody (60335-1-Ig)

    IF Staining of human breast cancer using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using E-cadherin Monoclonal antibody (60335-1-Ig)

    IF Staining of MCF-7 using 60335-1-Ig (same clone as 60335-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using E-cadherin antibody (60335-1-Ig, Clone: 6B11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60335-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60335-1-PBS targets E-cadherin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E-cadherin fusion protein Ag15085 相同性解析による交差性が予測される生物種
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC141838
Gene symbol E-cadherin
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.