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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E-cadherin Recombinant antibody

E-cadherin Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240159A1

Cat no : 80541-7-RR

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using E-cadherin Recombinant antibody (80541-7-RR)

    WB analysis using 80541-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80541-7-RR (E-cadherin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using E-cadherin Recombinant antibody (80541-7-RR)

    IF Staining of MCF-7 using 80541-7-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-7-RR, Clone: 240159A1 ) at dilution of 1:160 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inCaco-2 cells, A431 cells, T-47D cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 80541-7-RR is E-cadherin Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen E-cadherin fusion protein Ag14973 相同性解析による交差性が予測される生物種
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Calculated molecular weight 882 aa, 97 kDa
Observed molecular weight135 kDa
GenBank accession numberBC141838
Gene symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

Protocols

Product Specific Protocols
WB protocol for E-cadherin antibody 80541-7-RRDownload protocol
IF protocol for E-cadherin antibody 80541-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols