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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E-selectin/CD62E Polyclonal antibody, PBS Only

E-selectin/CD62E Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20894-1-PBS

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20894-1-PBS targets E-selectin/CD62E in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-selectin/CD62E fusion protein Ag14944 相同性解析による交差性が予測される生物種
Full Name selectin E
Calculated molecular weight 610 aa, 67 kDa
GenBank accession numberBC142711
Gene symbol CD62E
Gene ID (NCBI) 6401
RRIDAB_10755287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)