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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E-selectin/CD62E Polyclonal antibody, PBS Only

E-selectin/CD62E Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20894-1-PBS

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human tonsillitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20894-1-AP (E-selectin / CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human appendicitis tissue using E-selectin/CD62E Polyclonal antibody (20894-1-AP)

    IHC staining of human appendicitis using 20894-1-AP (same clone as 20894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 20894-1-AP (E-selectin /CD62E antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20894-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20894-1-PBS targets E-selectin/CD62E in IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-selectin/CD62E fusion protein Ag14944 相同性解析による交差性が予測される生物種
Full Name selectin E
Calculated molecular weight 610 aa, 67 kDa
GenBank accession numberBC142711
Gene symbol CD62E
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)