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  • Featured Product
  • KD/KO Validated

E2F1 Monoclonal antibody, PBS Only

E2F1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5D7G8

Cat no : 66515-1-PBS

Synonyms

RBBP-3, RBAP-1, pRB-binding protein E2F-1, E2F-1, E2F 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using E2F1 Monoclonal antibody (66515-1-Ig)

    WB analysis using 66515-1-Ig (same clone as 66515-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66515-1-Ig (E2F1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using E2F1 Monoclonal antibody (66515-1-Ig)

    WB analysis of HepG2 using 66515-1-Ig (same clone as 66515-1-PBS)

    WB result of E2F1 antibody (66515-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-E2F1 transfected HepG2 cells. This data was developed using the same antibody clone with 66515-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66515-1-PBS targets E2F1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen E2F1 fusion protein Ag17363 相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 1
Calculated molecular weight 437 aa, 47 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC050369
Gene symbol E2F1
Gene ID (NCBI) 1869
RRIDAB_2881878
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Transcription factor E2F1 (E2F1), also known as RBBP3, is a transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The calculated molecular weight of E2F1 is 47 kDa, but the sumoylated E2F1 is bout 55-60 kDa.