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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E2F6 Polyclonal antibody

E2F6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 31464-1-AP

Synonyms

E2F-6, E2F 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using E2F6 Polyclonal antibody (31464-1-AP)

    WB analysis using 31464-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31464-1-AP (E2F6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using E2F6 Polyclonal antibody (31464-1-AP)

    IF Staining of Jurkat using 31464-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using E2F6 antibody (31464-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31464-1-AP targets E2F6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F6 fusion protein Ag35112 相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 6
Calculated molecular weight 32 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC008348
Gene symbol E2F6
Gene ID (NCBI) 1876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

E2F6 is a member of the E2F family of TFs that control cell proliferation and cell fate. Cancer patients with high E2F6 expression in tumors, such as glioblastoma, breast cancer, and hepatocellular carcinoma, have poor prognosis.

Protocols

Product Specific Protocols
WB protocol for E2F6 antibody 31464-1-APDownload protocol
IF protocol for E2F6 antibody 31464-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols