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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

E2F7 Recombinant antibody, PBS Only

E2F7 Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230073C2

Cat no : 82887-1-PBS

Synonyms

Transcription factor E2F7, E2F-7, 230073C2

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using E2F7 Recombinant antibody (82887-1-RR)

    IF Staining of HeLa using 82887-1-RR (same clone as 82887-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using E2F7 antibody (82887-1-RR, Clone: 230073C2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82887-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using E2F7 Recombinant antibody (82887-1-RR)

    FC experiment of HeLa using 82887-1-RR (same clone as 82887-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug E2F7 Recombinant antibody (82887-1-RR, Clone:230073C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82887-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82887-1-RR

    Affinity and Kinetic Characterization of 82887-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82887-1-RR against Human E2F7 were performed. The affinity constant is 31.3 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82887-1-PBS targets E2F7 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen E2F7 fusion protein Ag21599 相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 7
Calculated molecular weight 911 aa, 100 kDa
GenBank accession numberBC136366
Gene symbol E2F7
Gene ID (NCBI) 144455
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

E2F7 also named as E2F transcription factor 7 is a 911 amino acid protein, which belongs to the E2F/DP family. E2F7 localizes in the nucleus and mainly forms homodimers and, to a lesser extent, heterodimers with E2F8. E2F7 as a transcription factor involves in various processes such as angiogenesis, polyploidization of specialized cells and DNA damage response. E2F7 mainly forms homodimers and to a lesser extent, heterodimers with E2F8. (PMID: 18194653 )