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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using E2F8 Polyclonal antibody (13425-1-AP)

    WB analysis of HeLa using 13425-1-AP (same clone as 13425-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using E2F8 Polyclonal antibody (13425-1-AP)

    WB analysis of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13425-1-AP (E2F8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using E2F8 Polyclonal antibody (13425-1-AP)

    IP experiment of HEK-293 using 13425-1-AP (same clone as 13425-1-PBS)

    IP result of anti-E2F8 (IP:13425-1-AP, 4ug; Detection:13425-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 13425-1-PBS in a different storage buffer formulation.

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E2F8 Polyclonal antibody, PBS Only

E2F8 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13425-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13425-1-PBS targets E2F8 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E2F8 fusion protein Ag4216 相同性解析による交差性が予測される生物種
Full Name E2F transcription factor 8
Calculated molecular weight 867 aa, 94 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC028244
Gene Symbol E2F8
Gene ID (NCBI) 79733
RRIDAB_2097273
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA0AVK6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

E2F8 is one E2F transcription factor that is essential for orchestrating expression of genes required for cell cycle progression, proliferation, apoptosis and differentiation. E2F8 shows a high degree of resemblance to E2F7 and shares the unique structure of E2F7 by having two distinct domains exhibiting a high degree of similarity to the DNA-binding domain of the E2F familyTogether with E2F7, they possess two DNA-binding domains that are predicted to interact with each other. E2F8 binds consensus E2F sites in a DP-independent manner and represses transcription of E2F-regulated promoters. Ectopic expression of E2F8 inhibits cellular proliferation. The calcualted molecular weight of E2F8 is 94 kDa, but modified E2F8 is about 105 kDa. (PMID: 15897886)