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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EAAT2 Monoclonal antibody (67083-1-Ig)

    WB analysis using 67083-1-Ig (same clone as 67083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67083-1-Ig (SLC1A2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EAAT2 Monoclonal antibody (67083-1-Ig)

    IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using EAAT2 Monoclonal antibody (67083-1-Ig)

    IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using EAAT2 Monoclonal antibody (67083-1-Ig)

    IHC staining of rat brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EAAT2 Monoclonal antibody (67083-1-Ig)

    IHC staining of mouse brain using 67083-1-Ig (same clone as 67083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67083-1-Ig (EAAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67083-1-PBS in a different storage buffer formulation.

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EAAT2 Monoclonal antibody, PBS Only

EAAT2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67083-1-PBS
Clone No.1C8D10

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

EAAT2, GLT 1, GLT1, SLC1A2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67083-1-PBS targets EAAT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EAAT2 fusion protein Ag19816 相同性解析による交差性が予測される生物種
Full Name solute carrier family 1 (glial high affinity glutamate transporter), member 2
Calculated molecular weight 574 aa, 62 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC132768
Gene Symbol EAAT2
Gene ID (NCBI) 6506
RRIDAB_2882391
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43004
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

EAAT2 (also known as GLT-1), is encoded by SLC1A2 and is a glutamate transporter which can clear the majority of extracellular glutamate in brain and prevent brain seizures.Three isoforms of EAAT2 exist due to the alternative splicing. In addition to the 65-70 kDa monomer form, 130-150 kDa dimer of EAAT2 can also be observed on SDS-PAGE.(PMID: 22114258)