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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EAF6 Polyclonal antibody

EAF6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26465-1-AP

Synonyms

C1orf149, CDABP0189, Eaf6, hEAF6, MEAF6, MYST/Esa1 associated factor 6, NY SAR 91, Protein EAF6 homolog, Sarcoma antigen NY SAR 91

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using EAF6 Polyclonal antibody (26465-1-AP)

    WB analysis of HepG2 using 26465-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EAF6 Polyclonal antibody (26465-1-AP)

    WB analysis of HeLa using 26465-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using EAF6 Polyclonal antibody (26465-1-AP)

    WB analysis of Jurkat using 26465-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26465-1-AP (EAF6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using EAF6 Polyclonal antibody (26465-1-AP)

    IP experiment of HepG2 using 26465-1-AP

    IP result of anti-EAF6 (IP:26465-1-AP, 4ug; Detection:26465-1-AP 1:500) with HepG2 cells lysate 3480ug.

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells
Positive IP detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 26465-1-AP is EAF6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EAF6 fusion protein Ag23971 相同性解析による交差性が予測される生物種
Full Name chromosome 1 open reading frame 149
Observed molecular weight 22-28 kDa
GenBank accession numberBC016328
Gene symbol MEAF6
Gene ID (NCBI) 64769
RRIDAB_2880524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EAF6 antibody 26465-1-APDownload protocol
IP protocol for EAF6 antibody 26465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Discov

MOZ and Menin-MLL Complexes are Complementary Regulators of Chromatin Association and Transcriptional Output in Gastrointestinal Stromal Tumor.

Authors - Matthew L Hemming
View Article
mouseWB

Cell Death Differ

MACF1 promotes osteoblast differentiation by sequestering repressors in cytoplasm.

Authors - Lifang Hu
View Article