• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EAPP Polyclonal antibody

EAPP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16931-1-AP

Synonyms

E2F-associated phosphoprotein, E2F associated phosphoprotein, C14orf11, BM-036, BM036

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using EAPP Polyclonal antibody (16931-1-AP)

    WB analysis of HEK-293 using 16931-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EAPP Polyclonal antibody (16931-1-AP)

    WB analysis of HeLa using 16931-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16931-1-AP (EAPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EAPP Polyclonal antibody (16931-1-AP)

    IF Staining of HeLa using 16931-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16931-1-AP (EAPP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16931-1-AP targets EAPP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EAPP fusion protein Ag10403 相同性解析による交差性が予測される生物種
Full Name E2F-associated phosphoprotein
Calculated molecular weight 285 aa, 33 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC001245
Gene symbol EAPP
Gene ID (NCBI) 55837
RRIDAB_2878334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EAPP antibody 16931-1-APDownload protocol
IF protocol for EAPP antibody 16931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols