Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver mitochondria tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17539-1-AP targets EARS2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EARS2 fusion protein Ag11594 相同性解析による交差性が予測される生物種 |
| Full Name | glutamyl-tRNA synthetase 2, mitochondrial (putative) |
| Calculated molecular weight | 534 aa, 60 kDa |
| Observed molecular weight | 59 kDa |
| GenBank accession number | BC040013 |
| Gene Symbol | EARS2 |
| Gene ID (NCBI) | 124454 |
| RRID | AB_2878414 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JPH6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EARS2 encodes mitochondrial glutamyltRNA synthetase 2. Two isoforms were produced by alternative splicing with predicted MW of 58 and 60 kDa according to UniProt, and this antibody recognises both.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EARS2 antibody 17539-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |