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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EBP50/NHERF-1 Polyclonal antibody

EBP50/NHERF-1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 29771-1-AP

Synonyms

SLC9A3R1, NHERF-1, EBP50

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    WB analysis using 29771-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:10000 and incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of human placenta using 29771-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of human placenta using 29771-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of rat kidney using 29771-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IHC staining of rat kidney using 29771-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 29771-1-AP (EBP50/NHERF-1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    IF Staining of HeLa using 29771-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EBP50/NHERF-1 antibody (29771-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using EBP50/NHERF-1 Polyclonal antibody (29771-1-AP)

    FC experiment of HepG2 using 29771-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-HUMAN EBP50/NHERF-1 (29771-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHCT 116 cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells, rat kidney tissue, mouse kidney tissue
Positive IHC detected inhuman placenta tissue, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29771-1-AP targets EBP50/NHERF-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EBP50/NHERF-1 fusion protein Ag30878 相同性解析による交差性が予測される生物種
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
Calculated molecular weight 39 kDa
Observed molecular weight 50 kDa
GenBank accession numberNM_004252
Gene symbol SLC9A3R1/EBP50/NHERF-1
Gene ID (NCBI) 9368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Na+/H+ exchanger regulatory factor 1 (NHERF-1, also known as EBP50 ezrin-binding protein of 50 kDa, SLCA9A3R1) is a phosphoprotein that assembles multiprotein complexes via two PDZ domains and a C-terminal ezrin-binding domain. NHERF-1 was initially recognized as a scaffolding protein that recruits membrane transporters/receptors and cytoplasmic signaling proteins into functional complexes localized at or near the plasma membrane in epithelial cells (PMID: 19308292).

Protocols

Product Specific Protocols
WB protocol for EBP50/NHERF-1 antibody 29771-1-APDownload protocol
IHC protocol for EBP50/NHERF-1 antibody 29771-1-APDownload protocol
IF protocol for EBP50/NHERF-1 antibody 29771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols