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  • Featured Product
  • KD/KO Validated

ECD Polyclonal antibody

ECD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10192-1-AP

Synonyms

Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ECD Polyclonal antibody (10192-1-AP)

    WB analysis of HeLa using 10192-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ECD Polyclonal antibody (10192-1-AP)

    WB analysis of HeLa using 10192-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ECD Polyclonal antibody (10192-1-AP)

    WB analysis of HeLa using 10192-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ECD Polyclonal antibody (10192-1-AP)

    WB analysis of HeLa using 10192-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10192-1-AP (ECD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ECD Polyclonal antibody (10192-1-AP)

    IP experiment of HeLa using 10192-1-AP

    IP result of anti-ECD (IP:10192-1-AP, 4ug; Detection:10192-1-AP 1:300) with HeLa cells lysate 2480ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ECD Polyclonal antibody (10192-1-AP)

    IHC staining of human ovary cancer using 10192-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10192-1-AP (ECD antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ECD Polyclonal antibody (10192-1-AP)

    IF Staining of HeLa using 10192-1-AP

    Immunofluorescent analysis of HeLa cells using 10192-1-AP (ECD antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10192-1-AP targets ECD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECD fusion protein Ag0245 相同性解析による交差性が予測される生物種
Full Name ecdysoneless homolog (Drosophila)
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC000721
Gene symbol ECD
Gene ID (NCBI) 11319
RRIDAB_2188183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human suppressor of GCR two (hSGT1) was isolated by its ability to complement the growth defect of the gcr2 mutant of Saccharomyces cerevisiae. Further work confirmed that the rescue of growth was associated with recovery of glycolytic enzyme activities of gcr2, and that hSGT1 did not complement the growth defect of a gcr1 mutant. A hybrid protein comprising hSgt1p and the DNA-binding domain of Gal4p (GBD) activated a GAL1-lacZ reporter gene fusion, suggesting that the cloned gene may be a transcriptional activator. Two-hybrid experiments in yeast also indicate that hSgt1p interacts with Gcr1p. Northern analysis showed that hSGT1 is highly expressed in muscle and heart.

Protocols

Product Specific Protocols
WB protocol for ECD antibody 10192-1-APDownload protocol
IHC protocol for ECD antibody 10192-1-APDownload protocol
IF protocol for ECD antibody 10192-1-APDownload protocol
IP protocol for ECD antibody 10192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Amplification of ACK1 promotes gastric tumorigenesis via ECDdependent p53 ubiquitination degradation.

Authors - Song-Hui Xu
  • KD Validated
View Article