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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECD Monoclonal antibody

ECD Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5E8

Cat no : 67179-1-Ig

Synonyms

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECD Monoclonal antibody (67179-1-Ig)

    WB analysis using 67179-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67179-1-Ig (ECD antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human renal cell carcinoma using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human renal cell carcinoma using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human stomach cancer using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of human stomach cancer using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of mouse bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of mouse bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat colon using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat colon tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat colon using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat bladder tissue using ECD Monoclonal antibody (67179-1-Ig)

    IHC staining of rat bladder using 67179-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 67179-1-Ig (ECD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, JAR cells, NCCIT cells, HEK-293 cells
Positive IHC detected inhuman renal cell carcinoma tissue, human stomach cancer tissue, mouse bladder tissue, rat colon tissue, rat bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67179-1-Ig targets ECD in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ECD fusion protein Ag28674 相同性解析による交差性が予測される生物種
Full Name ecdysoneless homolog (Drosophila)
Calculated molecular weight 73 kDa
Observed molecular weight 70-73 kDa
GenBank accession numberBC000721
Gene symbol ECD
Gene ID (NCBI) 11319
RRIDAB_2882475
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ECD antibody 67179-1-IgDownload protocol
IHC protocol for ECD antibody 67179-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols