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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26088-1-AP

Synonyms

ECE, ECE 1, ECE1, endothelin converting enzyme 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECE1 Polyclonal antibody (26088-1-AP)

    WB analysis using 26088-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ECE1 Polyclonal antibody (26088-1-AP)

    WB analysis of A549 using 26088-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ECE1 Polyclonal antibody (26088-1-AP)

    WB analysis of HeLa using 26088-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26088-1-AP (ECE1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ECE1 Polyclonal antibody (26088-1-AP)

    IHC staining of human breast cancer using 26088-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ECE1 Polyclonal antibody (26088-1-AP)

    IHC staining of human breast cancer using 26088-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26088-1-AP (ECE1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26088-1-AP is ECE1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECE1 fusion protein Ag23144 相同性解析による交差性が予測される生物種
Full Name endothelin converting enzyme 1
Observed molecular weight 125-135 kDa
GenBank accession numberBC126257
Gene symbol ECE1
Gene ID (NCBI) 1889
RRIDAB_2880371
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ECE1 antibody 26088-1-APDownload protocol
IHC protocol for ECE1 antibody 26088-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Laryngoscope Investig Otolaryngol

Hypoxia-inducible factor-1α contributes to the proliferation of cholesteatoma keratinocytes through regulating endothelin converting enzyme 1 expression

Authors - Nie Chen
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Jaouhara (Verified Customer) (12-05-2023)

Specific antibody. Validated in immunofluorescence and immunohistochemistry. Good signal.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Cell Tissue Type: Chondrocytes