Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.