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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECH1 Monoclonal antibody, PBS Only

ECH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 68139-1-PBS

Synonyms

ECH1, HPXEL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECH1 Monoclonal antibody (68139-1-Ig)

    WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ECH1 Monoclonal antibody (68139-1-Ig)

    IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ECH1 Monoclonal antibody (68139-1-Ig)

    IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ECH1 Monoclonal antibody (68139-1-Ig)

    IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68139-1-PBS targets ECH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ECH1 fusion protein Ag32661 相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Calculated molecular weight 328 aa, 36 kDa
Observed molecular weight 33-36 kDa
GenBank accession numberBC017408
Gene symbol ECH1
Gene ID (NCBI) 1891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.