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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECHDC3 Polyclonal antibody

ECHDC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27098-1-AP

Synonyms

PP8332, PP1494, Enoyl-CoA hydratase domain-containing protein 3, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECHDC3 Polyclonal antibody (27098-1-AP)

    WB analysis using 27098-1-AP

    MDCK lysates were subjected to SDS PAGE followed by western blot with 27098-1-AP (ECHDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using ECHDC3 Polyclonal antibody (27098-1-AP)

    IHC staining of human ovary cancer using 27098-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27098-1-AP (ECHDC3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMDCK cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27098-1-AP targets ECHDC3 in WB, IHC, ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECHDC3 fusion protein Ag25707 相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase domain containing 3
Observed molecular weight 33 kDa
GenBank accession numberBC009617
Gene symbol ECHDC3
Gene ID (NCBI) 79746
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ECHDC3 antibody 27098-1-APDownload protocol
IHC protocol for ECHDC3 antibody 27098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols