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  • Featured Product
  • KD/KO Validated

ECHS1 Monoclonal antibody, PBS Only

ECHS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B9D5

Cat no : 66117-1-PBS

Synonyms

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ECHS1 Monoclonal antibody (66117-1-Ig)

    WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB result of ECHS1 antibody (66117-1-Ig, 1:20,000) with si-Control and si-ECHS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ECHS1 Monoclonal antibody (66117-1-Ig)

    WB analysis of MCF-7 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB result of ECHS1 antibody (66117-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ECHS1 Monoclonal antibody (66117-1-Ig)

    WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    WB analysis of human testis using 66117-1-Ig (same clone as 66117-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using ECHS1 Monoclonal antibody (66117-1-Ig)

    WB analysis of PC-3 using 66117-1-Ig (same clone as 66117-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using ECHS1 Monoclonal antibody (66117-1-Ig)

    IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    IP result of anti-ECHS1 (IP:66117-1-Ig, 3ug; Detection:66117-1-Ig 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    IHC staining of human liver using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ECHS1 Monoclonal antibody (66117-1-Ig)

    IHC staining of human liver using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66117-1-Ig (ECHS1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ECHS1 Monoclonal antibody (66117-1-Ig)

    IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (66117-1-Ig, Clone: 2B9D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66117-1-PBS targets ECHS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ECHS1 fusion protein Ag16775 相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC008906
Gene symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)