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  • Featured Product
  • KD/KO Validated

ECHS1 Recombinant antibody

ECHS1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5G5

Cat no : 81611-1-RR

Synonyms

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ECHS1 Recombinant antibody (81611-1-RR)

    WB analysis of MCF-7 using 81611-1-RR

    WB result of ECHS1 antibody (81611-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using ECHS1 Recombinant antibody (81611-1-RR)

    WB analysis using 81611-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ECHS1 Recombinant antibody (81611-1-RR)

    WB analysis using 81611-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using ECHS1 Recombinant antibody (81611-1-RR)

    IHC staining of human prostate cancer using 81611-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ECHS1 Recombinant antibody (81611-1-RR)

    IHC staining of human colon using 81611-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ECHS1 Recombinant antibody (81611-1-RR)

    IF Staining of HeLa using 81611-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 81611-1-RR

    Affinity and Kinetic Characterization of 81611-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81611-1-RR against Human ECHS1 were performed. The affinity constant is 95.5 pM.

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Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, mouse liver tissue, L02 cells, Caco-2 cells, HepG2 cells, HEK-293 cells, PC-3 cells, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81611-1-RR targets ECHS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ECHS1 fusion protein Ag1842 相同性解析による交差性が予測される生物種
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Calculated molecular weight 31 kDa
Observed molecular weight30 kDa
GenBank accession numberBC008906
Gene symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

Protocols

Product Specific Protocols
WB protocol for ECHS1 antibody 81611-1-RRDownload protocol
IHC protocol for ECHS1 antibody 81611-1-RRDownload protocol
IF protocol for ECHS1 antibody 81611-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols