Validation Data Gallery
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Tested Applications
| Positive WB detected in | Transfected HEK-293 cells |
| Positive IHC detected in | human kidney tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
12611-1-AP targets ECOP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ECOP fusion protein Ag3225 相同性解析による交差性が予測される生物種 |
| Full Name | EGFR-coamplified and overexpressed protein |
| Calculated molecular weight | 172 aa, 19 kDa |
| GenBank accession number | BC016650 |
| Gene Symbol | ECOP |
| Gene ID (NCBI) | 81552 |
| RRID | AB_2877866 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AW1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ECOP, also named as VOPP1 and GASP, has been previously shown to be over-expressed in human glioblastoma multiforme and squamous cell carcinoma. It interacts directly with cytoplasmic mediators of the NF kappa B pathway. ECOP has three isoforms with MW 18-19 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ECOP antibody 12611-1-AP | Download protocol |
| IHC protocol for ECOP antibody 12611-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Biol VOPP1 promotes breast tumorigenesis by interacting with the tumor suppressor WWOX.
| ||
Aging (Albany NY) Plumbagin has an inhibitory effect on the growth of TSCC PDX model and it enhances the anticancer efficacy of cisplatin |