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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ECT2 Polyclonal antibody

ECT2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 26557-1-AP

Synonyms

Protein ECT2, Epithelial cell-transforming sequence 2 oncogene

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ECT2 Polyclonal antibody (26557-1-AP)

    WB analysis using 26557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26557-1-AP (ECT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ECT2 Polyclonal antibody (26557-1-AP)

    WB analysis using 26557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26557-1-AP (ECT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using ECT2 Polyclonal antibody (26557-1-AP)

    IP experiment of K-562 using 26557-1-AP

    IP result of anti-ECT2 (IP:26557-1-AP, 4ug; Detection:26557-1-AP 1:6000) with K-562 cells lysate 2400 ug.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ECT2 Polyclonal antibody (26557-1-AP)

    IF Staining of A549 using 26557-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ECT2 antibody (26557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ECT2 Polyclonal antibody (26557-1-AP)

    IF Staining of NIH/3T3 using 26557-1-AP

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ECT2 antibody (26557-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A549 cells using ECT2 Polyclonal antibody (26557-1-AP)

    FC experiment of A549 using 26557-1-AP

    1x10^6 A549 cells were intracellularly stained with 0.25 ug ECT2 Polyclonal antibody (26557-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inA549 cells, HEK-293T cells, K-562 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inA549 cells, NIH/3T3 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26557-1-AP targets ECT2 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ECT2 fusion protein Ag24836 相同性解析による交差性が予測される生物種
Full Name epithelial cell transforming sequence 2 oncogene
Observed molecular weight 100 kDa
GenBank accession numberBC112086
Gene symbol ECT2
Gene ID (NCBI) 1894
RRIDAB_3669547
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Epithelial cell transforming sequence 2 (Ect2) is a guanine nucleotide exchange factor (GEF) for the Rho family GTPases, Rac1, RhoA and Cdc42. Ect2 catalyzes the exchange of GDP for GTP, thereby activating the Rho GTPases toward their downstream effectors  (PMID: 35069543). The cellular localization of Ect2 is cell cycle dependent. Ect2 is localized predominantly in the nucleus during interphase due to two functional NLS sequences found within its central hinge region.1 At the onset of mitosis, Ect2 is dispersed throughout the cytoplasm where it associates with the mitotic spindle at metaphase, the cleavage furrow during telophase, and the mid-body during cytokinesis (PMID: 35069543). Besides normal cellular activity, ECT2 also participates in malignant transformation, tumor initiation, and metastasis (PMID: 36572949).

Protocols

Product Specific Protocols
WB protocol for ECT2 antibody 26557-1-APDownload protocol
IF protocol for ECT2 antibody 26557-1-APDownload protocol
IP protocol for ECT2 antibody 26557-1-APDownload protocol
FC protocol for ECT2 antibody 26557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols