EDC3 Polyclonal antibody
EDC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 16486-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human heart tissue, human placenta tissue, human skin tissue, human liver tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 3 publications below |
Product Information
16486-1-AP targets EDC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EDC3 fusion protein Ag9608 相同性解析による交差性が予測される生物種 |
| Full Name | enhancer of mRNA decapping 3 homolog (S. cerevisiae) |
| Calculated molecular weight | 508 aa, 56 kDa |
| Observed molecular weight | 56 kDa |
| GenBank accession number | BC021271 |
| Gene symbol | EDC3 |
| Gene ID (NCBI) | 80153 |
| RRID | AB_2095946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EDC3 antibody 16486-1-AP | Download protocol |
| IHC protocol for EDC3 antibody 16486-1-AP | Download protocol |
| IF protocol for EDC3 antibody 16486-1-AP | Download protocol |
| IP protocol for EDC3 antibody 16486-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition | ||
PLoS Genet Two RNA binding proteins, ADAD2 and RNF17, interact to form a heterogeneous population of novel meiotic germ cell granules with developmentally dependent organelle association | ||
Cell Rep Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes. | ||
Nucleic Acids Res RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Aditya (Verified Customer) (11-01-2024) | great with ecl
|
