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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EDEM3 Polyclonal antibody

EDEM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27310-1-AP

Synonyms

ER degradation-enhancing alpha-mannosidase-like protein 3, EC:3.2.1.113, C1orf22, Alpha-1,2-mannosidase EDEM3

Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using EDEM3 Polyclonal antibody (27310-1-AP)

    WB analysis of 3T3-L1 using 27310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27310-1-AP (EDEM3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using EDEM3 Polyclonal antibody (27310-1-AP)

    IP experiment of HEK-293 using 27310-1-AP

    IP result of anti-EDEM3 (IP:27310-1-AP, 4ug; Detection:27310-1-AP 1:300) with HEK-293 cells lysate 2600ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using EDEM3 Polyclonal antibody (27310-1-AP)

    IHC staining of human stomach cancer using 27310-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27310-1-AP (EDEM3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected in3T3-L1 cells, HEK-293 cell
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27310-1-AP targets EDEM3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EDEM3 fusion protein Ag26123 相同性解析による交差性が予測される生物種
Full Name ER degradation enhancer, mannosidase alpha-like 3
Calculated molecular weight 105 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC016464
Gene symbol EDEM3
Gene ID (NCBI) 80267
RRIDAB_2880839
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EDEM3 antibody 27310-1-APDownload protocol
IHC protocol for EDEM3 antibody 27310-1-APDownload protocol
IP protocol for EDEM3 antibody 27310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Emma (Verified Customer) (03-15-2022)

Works well (single band) by WB in LNCaP and CWR22Rv1 cells @ 1:1000

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate