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EEA1 Polyclonal antibody

EEA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28347-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Cell and organelle markers, early endosome antigen 1, EEA1, Endosome Marker, MST105, MSTP105, ZFYVE2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, HepG2 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 7 publications below

Product Information

28347-1-AP targets EEA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EEA1 fusion protein Ag28814 相同性解析による交差性が予測される生物種
Full Name early endosome antigen 1
Calculated molecular weight 162 kDa
Observed molecular weight 170 kDa
GenBank accession numberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
RRIDAB_2881117
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15075
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

Protocols

Product Specific Protocols
WB protocol for EEA1 antibody 28347-1-APDownload protocol
IHC protocol for EEA1 antibody 28347-1-APDownload protocol
IF protocol for EEA1 antibody 28347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nucleic Acids Res

Nucleosomes enter cells by clathrin- and caveolin-dependent endocytosis.

Authors - Huawei Wang
mouseIF

Front Immunol

Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.

Authors - Yafeng Dou
humanIF

Chemistry

Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

Authors - Sayan Kundu
humanIF

Cell Death Dis

SorCS3 promotes the internalization of p75NTR to inhibit GBM progression.

Authors - Yanqiu Zhang
monkeyIF

Toxicol Lett

Anti-ricin toxin human neutralizing antibodies and DMAbs protection against ricin toxin poisoning

Authors - Haotian Yu
mouseIF

Nat Commun

Sortilin-mediated translocation of mitochondrial ACSL1 impairs adipocyte thermogenesis and energy expenditure in male mice

Authors - Min Yang
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